LMB / Science / Facilities / Flow Cytometry
Pier Andrée Penttilä

Contact

Facilities

Flow Cytometry

The Flow Cytometry facility provides bespoke cytometry methods with an emphasis on synergistic collaboration across specialisations. This includes high-dimensional spectral immunophenotyping, human chromosome isolation, identification and quantification of DNA synthesis and degradation, single cell analysis and isolation of rare cells, CRISPR-mediated genetic screening and purification of cells and particles for downstream studies using light microscopy, electron cryomicroscopy or mass spectrometry. The facility works closely alongside the Biological Services Group to generate quantitative and qualitative reproducible data from transgenic mouse strains.

Two scientists, one female and one male, wearing white lab coats, purple nitrile gloves and safety glasses, standing in front of a cell sorting instrument displaying two-dimensional flow cytometry dot plots and droplet generation on a computer screen.  The male scientist is holding a 5 ml polypropylene tube containing a turbid single cell suspension.  The female scientist is pointing to the bottom of the tube to indicate to the male scientist where cell aggregates are likely to form.
Sorting of cell suspensions in the Flow Cytometry facility.

Equipment includes five cell sorters; one 5-laser Sony Synergy system, two 7-laser ThermoFisher Bigfoots, a 6-laser quartz-cuvette BD FACSAria Fusion and a 4-laser Miltenyi Tyto Lux system. LMB scientists also have access to five analysers: two BD LSR Fortessas, one Beckman Coulter CytoFLEX and two 6-laser Sony ID7000 spectral cytometers. One of the ID7000 instruments is equipped with an automated Hudson robotic arm, capable of running up to 16 plates for high-throughput screening.

Sony flow cytometer and computer displaying colorful cell analysis data, with additional lab equipment and wall art in a bright room.
Spectral flow cytometer instrument coupled to a robotic arm that acquires 96 well microtiter plates containing cells.

Selected Publications

ILC2-derived LIF licences progress from tissue to systemic immunity.Gogoi M, Clark PA, Ferreira ACF, Rodriguez Rodriguez N, Heycock M, Ko M, Murphy JE, Chen V, Luan SL, Jolin HE, McKenzie ANJNature 632(8026): 885-892 (2024)
Mef2d potentiates type-2 immune responses and allergic lung inflammation.Szeto ACH, Clark PA, Ferreira ACF, Heycock M, Griffiths EL, Jou E, Mannion J, Luan SL, Storrar S, Knolle MD, Kozik P, Jolin HE, Fallon PG, McKenzie ANJScience 384(6703): eadl0370 (2024)
Perforin-2 is a pore-forming effector of endocytic escape in cross-presenting dendritic cells.Rodríguez-Silvestre P, Laub M, Krawczyk PA, Davies AK, Schessner JP, Parveen R, Tuck BJ, McEwan WA, Borner GHH, Kozik PScience 380(6651): 1258-1265 (2023)
The patterned assembly and stepwise Vps4-mediated disassembly of composite ESCRT-III polymers drives archaeal cell division.Hurtig F, Burgers TCQ, Cezanne A, Jiang X, Mol FN, Traparić J, Pulschen AA, Nierhaus T, Tarrason-Risa G, Harker-Kirschneck L, Löwe J, Šarić A, Vlijm R, Baum BSci Adv 9(11): eade5224 (2023)