Light Microscopy

The Light Microscopy facility provides expertise in quantitative biological imaging across multiple scales, from single-molecule techniques to tissue-wide analysis. The facility supports high-resolution imaging in live samples and develops bespoke image analysis methods in collaboration with scientists across the LMB.

The facility manages state-of-the-art microscopes optimised for a diverse range of techniques including: TIRF and single molecule fluorescence, confocal microscopy, super-resolution microscopy, including single molecule localisation, STED and structured illumination microscopy, batch slide scanning and plate-based high content microscopy and multiphoton microscopy. Many systems are configured for live sample imaging over extended periods and have been modified in-house to meet specific needs.

The facility enables quantitative image analysis by providing workstations with software for deconvolution, visualisation and advanced analysis. Comprehensive training and support for various commercial and open-source software is provided in-house. The facility also develops custom solutions for specific research projects, including neural network training and deployment, and it has an active research programme developing novel methods in image processing.

The facility works with users at all levels, from instruction in microscope use to advising on experiment design. It also works with groups to develop acquisition protocols and image analysis methods.