Published Research
Found 118 results for Scheres.
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Seeding biosensor cell line that reproduces the Alzheimer tau fold
AuthorsTaxiarchis Katsinelos, Sofia Lövestam, Chao Qi, Bejamin Ryskeldi-Falcon, Jennifer A. Macdonald, Gabriel Stephenson, Bernardino Ghetti, Ann McKee, Sjors H. W. Scheres, Michel Goedert.
Journal
The Journal of Biological Chemistry
301(12):110952 (2025)
Details
DOI10.1016/j.jbc.2025.110952 PMID41260337 PMCIDPMC12765094 DivisionStructural Studies, Neurobiology - Seed structure and phosphorylation in the fuzzy coat impact tau seeding competency AuthorsKasen A., Lövestam S., Breton L., Meyerdirk L., McPhail J.A., Piche K., Louwrier A., Capan C.D., Lee H., Scheres S.H.W., Henderson M.X. Journal Nature Communications 16(1):9240 (2025)
- Serial amplification of tau filaments using Alzheimer's brain homogenates and C322A or C322S recombinant tau AuthorsSantambrogio A., Lövestam S., Metrick 2nd M.A, Löhr T., Xu P., Gallagher N.C.T., Ghetti B., Caughey B., Scheres S.H.W, Vendruscolo M. Journal FEBS Letters 599(19):2768-2778 (2025)
- Twelve phosphomimetic mutations induce the assembly of recombinant full-length human tau into paired helical filaments AuthorsLövestam S, Wagstaff JL, Katsinelos T, Shi J, Freund SM, Goedert M, Scheres SH Journal eLife (2025)
- Cryo-EM structure of the vault from human brain reveals symmetry mismatch at its caps AuthorsLövestam S., Scheres S.H.W. Journal Structure :S0969-2126(25)00262-X (2025)
- GSK3β phosphorylated tau filaments are probably not like those from AD brains AuthorsScheres S.H.W. Journal Proceedings of the National Academy of Sciences of the United States of America 122(28):e2503730122 (2025)
- Distinct tau filament folds in human MAPT mutants P301L and P301T AuthorsSchweighauser M., Shi Y., Murzin A.G., Garringer H.J., Vidal R., Murrell J.R., Elena Erro M., Seelaar H., Ferrer I., van Swieten J.C., Ghetti B., Scheres S.H.W., Goedert M. Journal Nature Structural & Molecular Biology (2025)
- Identical seeding characteristics and cryo-EM filament structures in FTLD-Synuclein and typical multiple system atrophy AuthorsCullinane P.W., Yang Y., Chelban V., Yen Goh Y., Ebanks K., Curless T., Wrigley S., de Pablo-Fernández E., Holton J., Peak-Chew S., Franco C., Woerman A.L., Houlden H., Warner T.T., Scheres S.H.W., Goedert M., Jaunmuktane Z. Journal Neuropathology and Applied Neurobiology 51(2):e70013 (2025)
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Tau filaments with the Alzheimer fold in human MAPT mutants V337M and R406W
AuthorsQi C., Lövestam S., Murzin A.G., Peak-Chew S., Franco C., Bogdani M., Latimer C., Murrell J.R., Cullinane P.W., Jaunmuktane Z., Bird T.D., Ghetti B., Scheres S.H.W., Goedert M.
Journal
Nature Structural & Molecular Biology
32(7):1297-1304 (2025)
Details
DOI10.1038/s41594-025-01498-5 PMID40044789 PMCIDPMC12263442 DivisionStructural Studies, Neurobiology - Driving tau pathogenicity seeding - How fibril structure and post-translational modifications alter seeding capacity AuthorsKasen A. , Breton L. , Meyerdirk L. , Lövestam S. , McPhail J. , Louwrier A. , Scheres S. , Henderson M. Journal Alzheimer’s and Dementia 20 Suppl 8:e094584. (2025)
- TMEM106B amyloid filaments in the Biondi bodies of ependymal cells AuthorsGhetti B., Schweighauser M., Jacobsen M. H., Gray D., Bacioglu M., Murzin A. G., Glazier B. S., Katsinelos T., Vidal R., Newell K. L., Gao S., Garringer H. J., Spillantini M. G., Scheres S. H. W., Goedert M. Journal Acta Neuropathologica 148(1):60 (2024)
- An image processing pipeline for electron cryo-tomography in RELION-5 AuthorsBurt A, Toader B, Warshamanage R, von Kügelgen A, Pyle E, Zivanov J, Kimanius D, Bharat TAM, Scheres SHW. Journal FEBS Open bio 14(11):1788-1804 (2024)
- DynaMight: estimating molecular motions with improved reconstruction from cryo-EM images AuthorsSchwab J, Kimanius D, Burt A, Dendooven T, Scheres SHW. Journal Nature Methods 21(10):1855-1862 (2024)
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Cleaved TMEM106B forms amyloid aggregates in central and peripheral nervous systems
AuthorsBacioglu M, Schweighauser M, Gray D, Lövestam S, Katsinelos T, Quaegebeur A, van Swieten J, Jaunmuktane Z, Davies SW, Scheres SHW, Goedert M, Ghetti B, Spillantini MG.
Journal
Acta Neuropathologica Communications
Jun 17;12(1):99 (2024)
Details
DOI10.1186/s40478-024-01813‑z PMID38886865 PMCIDPMC11181561 DivisionStructural Studies , Neurobiology - Data-driven regularization lowers the size barrier of cryo-EM structure determination AuthorsKimanius D, Jamali K, Wilkinson ME, Lövestam S, Velazhahan V, Nakane T, Scheres SHW. Journal Nature Methods 10.1038/s41592-024-02304-8 (2024)
- Tau filaments with the chronic traumatic encephalopathy fold in a case of vacuolar tauopathy with VCP mutation D395G AuthorsQi C, Kobayashi R, Kawakatsu S, Kametani F, Scheres SHW, Goedert M, Hasegawa M. Journal Acta Neuropathologica 147(1):86 (2024)
- Community recommendations on cryoEM data archiving and validation AuthorsKleywegt GJ, Adams PD, Butcher SJ, Lawson CL, Rohou A, Rosenthal PB, Subramaniam S, Topf M, Abbott S, Baldwin PR, Berrisford JM, Bricogne G, Choudhary P, Croll TI, Danev R, Ganesan SJ, Grant T, Gutmanas A, Henderson R, Heymann JB, Huiskonen JT, Istrate A, Kato T, Lander GC, Lok SM, Ludtke SJ, Murshudov GN, Pye R, Pintilie GD, Richardson JS, Sachse C, Salih O, Scheres SHW, Schroeder GF, Sorzano COS, Stagg SM, Wang Z, Warshamanage R, Westbrook JD, Winn MD, Young JY, Burley SK, Hoch JC, Kurisu G, Morris K, Patwardhan A, Velankar S. Journal Iucrj 11(Pt 2):140-151 (2024)
- Automated model building and protein identification in cryo-EM maps AuthorsJamali K., Käll L., Zhang R., Brown A., Kimanius D., Scheres S. H. W. Journal Nature 628(8007):450-457 (2024)
- Disease-specific tau filaments assemble via polymorphic intermediates AuthorsLövestam S,. Li D., Wagstaff J. L., Kotecha A., Kimanius D., McLaughlin S. H., Murzin A. G., Freund S. M. V, Goedert M., Scheres S. H. W. Journal Nature 625(7993):119-125 (2024)
- Tau and neurodegeneration AuthorsGoedert M., Crowther R. A., Scheres S. H. W., Spillantini M. G. Journal Cytoskeleton 81(1):95-102 (2024)
- Cryo-EM structures of tau filaments from the brains of mice transgenic for human mutant P301S Tau AuthorsSchweighauser M., Murzin A. G., Macdonald J., Lavenir I., Crowther R. A., Scheres S. H. W., Goedert M. Journal Acta Neuropathologica Communications 11(1):160 (2023)
- Molecular pathology of neurodegenerative diseases by cryo-EM of amyloids AuthorsScheres S. H. W., Ryskeldi-Falcon B., Goedert M. Journal Nature 621(7980):701-710 (2023)
- Structures, distributions, and conformations of SARS-CoV-2 spike proteins on intact virions by Cryo-EM and Cryo‑ET AuthorsKe Z., Oton J., Qu K., Scheres S. H. W., Briggs J. A. G. Journal Microscopy and Microanalysis 29 (Supplement_1) 902–903 (2023)
- Cryo-EM structure of the complete inner kinetochore of the budding yeast point centromere AuthorsDendooven T., Zhang Z., Yang J., McLaughlin S. H., Schwab J., Scheres S. H. W., Yatskevich S., Barford D. Journal Science Advances 9(30):eadg7480 (2023)
- Cryo-EM structures of tau filaments from SH-SY5Y cells seeded with brain extracts from cases of Alzheimer's disease and corticobasal degeneration AuthorsTarutani A., Lövestam S., Zhang X., Kotecha A., Robinson A. C., Mann D. M. A., Saito Y., Murayama S., Tomita T., Goedert M., Scheres S. H. W., Hasegawa M. Journal FEBS Open bio 13(8):1394-1404 (2023)
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Mutation ∆K281 in MAPT causes Pick's disease
AuthorsSchweighauser M., Garringer H. J., Klingstedt T., Nilsson K. P. R., Masuda-Suzukake M., Murrell J. R., Risacher S L., Vidal R., Scheres S. H. W., Goedert M., Ghetti B., Newell K. L.
Journal
Acta Neuropathologica
146(2):211-226 (2023)
Details
DOIdoi.org/10.1007/s00401-023-02598‑6 PMID37351604 PMCIDPMC10329087 DivisionStructural Studies - Abundant Aβ fibrils in ultracentrifugal supernatants of aqueous extracts from Alzheimer's disease brains AuthorsStern A. M., Yang Y., Jin S., Yamashita K., Meunier A. L., Liu W., Cai Y., Ericsson M., Liu L., Goedert M., Scheres S. H. W., Selkoe D. J. Journal Neuron 111(13):2012-2020.e4 (2023)
- Identical tau filaments in subacute sclerosing panencephalitis and chronic traumatic encephalopathy AuthorsQi C., Hasegawa M., Takao M., Sakai M., Sasaki M., Mizutani M., Akagi A., Iwasaki Y., Miyahara H., Yoshida M., Scheres S. H. W., Goedert M. Journal Acta Neuropathologica Communications 11(1):74 (2023)
- New SNCA mutation and structures of α-synuclein filaments from juvenile-onset synucleinopathy AuthorsYang Y., Garringer H. J., Shi Y., Lövestam S., Peak-Chew S., Zhang X., Kotecha A., Bacioglu M., Koto A., Takao M., Spillantini M. G., Ghetti B., Vidal R., Murzin A. G., Scheres S. H. W., Goedert M. Journal Acta Neuropathologica 145(5):561-572 (2023)
- Cryo-EM structures of amyloid-β filaments with the Arctic mutation (E22G) from human and mouse brains AuthorsYang Y., Zhang W., Murzin A. G., Schweighauser M., Huang M., Lövestam S., Peak-Chew S. Y., Saito T., Saido T. C., Macdonald J., Lavenir I., Ghetti B., Graff C., Kumar A., Nordberg A., Goedert M., Scheres S. H. W. Journal Acta Neuropathologica 145(3):325-333 (2023)
- A Bayesian approach to single-particle electron cryo-tomography in RELION-4.0 AuthorsZivanov J, Otón J, Ke Z, von Kügelgen A, Pyle E, Qu K, Morado D, Castaño-Díez D, Zanetti G, Bharat TAM, Briggs JAG, Scheres SHW. Journal Elife Dec 5;11:e83724 (2022)
- Pushing the limits in single particle cryo-EM: general discussion AuthorsBakker SE, Bhella D, Brescia R, Bullough P, Clare DK, Daum B, Frank RAW, Gold VAM, Jackson Hirst I, Kühlbrandt W, Lu P, McLaren M, Menday R, Muench SP, Rauschenbach S, Russo CJ, Saibil H, Scheres SHW, Shah AR, Smith C, Torpey J, Zanetti G. Journal Faraday Discussions 10.1039/d2fd90063g (2022)
- Sample preparation in single particle cryo-EM: general discussion AuthorsAl-Otaibi N, Aminian J A, Anane RF, Baatsen P, Bakker SE, Bergeron J, Bharadwaj A, Bhella D, Braun T, Brescia R, Bullough P, Clare DK, Daum B, Esser TK, Farinas Lucas IDM, Frank RAW, Gold VAM, Harrison PJ, Hirst IJ, Klebl DP, Kühlbrandt W, Morton C, Muench SP, Nakasone M, Russo CJ, Saibil HR, Scheres SHW, Sehrawat V, Shah AR, Smith C, Thompson RF, Thorn A, Zanetti G. Journal Daraday Discussions 10.1039/d2fd90059a (2022)
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Structures of α-synuclein filaments from human brains with Lewy pathology
AuthorsYang Y., Shi Y., Schweighauser M., Zhang X., Kotecha A., Murzin A.G., Garringer H.J., Cullinane P.W., Saito Y., Foroud T., Warner T.T., Hasegawa K., Vidal R., Murayama S., Revesz T., Ghetti B., Hasegawa M., Lashley T., Scheres S.H.W., Goedert M.
Journal
Nature
610(7933):791-795 (2022)
Details
DOI10.1038/s41586-022-05319‑3 PMID36108674 PMCIDPMC7613749 DivisionStructural Studies, Neurobiology -
Age-dependent formation of TMEM106B amyloid filaments in human brains
AuthorsSchweighauser M., Arseni D., Bacioglu M., Huang M., Lövestam S., Shi Y., Yang Y., Zhang W., Kotecha A., Garringer H. J., Vidal R., Hallinan G. I., Newell K. L., Tarutani A., Murayama S., Miyazaki M., Saito Y., Yoshida M., Hasegawa K., Lashley T., Revesz T., Kovacs G. G., van Swieten J., Takao M., Hasegawa M., Ghetti B., Spillantini M. G., Ryskeldi-Falcon B., Murzin A. G., Goedert M., Scheres S. H. W.
Journal
Nature
605(7909):310-314 (2022)
Details
DOI10.1038/s41586-022-04650-z PMID35344985 PMCIDPMC9095482 DivisionNeurobiology, Structural Studies - High-throughput cryo-EM structure determination of amyloids AuthorsLövestam S, Scheres SHW. Journal Faraday Discussions Nov 8;240(0):243-260 (2022)
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Assembly of recombinant tau into filaments identical to those of Alzheimer's disease and chronic traumatic encephalopathy
AuthorsLövestam S., Koh F. A., van Knippenberg B., Kotecha A., Murzin A. G., Goedert M., Scheres S. H. W.
Journal
Elife
11:e76494 (2022)
Details
DOI10.7554/elife.76494 PMID35244536 PMCIDPMC8983045 DivisionNeurobiology, Structural Studies -
Cryo-EM structures of amyloid-β 42 filaments from human brains
AuthorsYang Y., Arseni D., Zhang W., Huang M., Lövestam S., Schweighauser M., Kotecha A., Murzin A. G., Peak-Chew S. Y., Macdonald J., Lavenir I., Garringer H. J., Gelpi E., Newell K. L., Kovacs G. G., Vidal R., Ghetti B., Ryskeldi-Falcon B., Scheres S. H. W., Goedert M.
Journal
Science
375(6577):167-172 (2022)
Details
DOI10.1126/science. Abm7285 PMID35025654 PMCIDPMC7612234 DivisionStructural Studies, Neurobiology - New tools for automated cryo-EM single-particle analysis in RELION-4.0 AuthorsKimanius D., Dong L., Sharov G., Nakane T., Scheres S. H. W. Journal Biochemical Journal 478(24):4169-4185 (2021)
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Structure-based classification of tauopathies
AuthorsShi Y., Zhang W., Yang Y., Murzin A. G., Falcon B., Kotecha A., van Beers M., Tarutani A., Kametani F., Garringer H. J., Vidal R., Hallinan G. I., Lashley T., Saito Y., Murayama S., Yoshida M., Tanaka H., Kakita A., Ikeuchi T., Robinson A. C., Mann D. M. A., Kovacs G. G., Revesz T., Ghetti B., Hasegawa M., Goedert M., Scheres S. H. W.
Journal
Nature
598(7880):359-363 (2021)
Details
DOI10.1038/s41586-021-03911-7 PMID34588692 PMCIDPMC7611841 DivisionNeurobiology, Structural Studies -
Correction to: Cryo-EM structures of tau filaments from Alzheimer's disease with PET ligand APN‑1607
AuthorsShi Y., Murzin A. G., Falcon B., Epstein A., Machin J., Tempest P., Newell K. L., Vidal R., Garringer H. J., Sahara N., Higuchi M., Ghetti B., Jang M. K., Scheres S. H. W., Goedert M.
Journal
Acta Neuropathologica
141(6):983. June 2021 (2021)
Details
DOI10.1007/s00401-021-02303-5 PMID33830331 PMCIDPMC8496564 DivisionStructural Studies, Neurobiology -
Seeded assembly in vitro does not replicate the structures of α-synuclein filaments from multiple system atrophy
AuthorsLövestam S., Schweighauser M., Matsubara T., Murayama S., Tomita T., Ando T., Hasegawa K., Yoshida M., Tarutani A., Hasegawa M., Goedert M., Scheres S. H. W.
Journal
FEBS Open bio
11(4):999-1013. April 2021 (2021)
Details
DOI10.1002/2211-5463.13110 PMID33548114 PMCIDPMC8016116 DivisionNeurobiology, Structural Studies -
Cryo-EM structures of tau filaments from Alzheimer's disease with PET ligand APN‑1607
AuthorsShi Y., Murzin A. G., Falcon B., Epstein A., Machin J., Tempest P., Newell K. L., Vidal R., Garringer H. J., Sahara N., Higuchi M., Ghetti B., Jang M. K., Scheres S. H. W., Goedert M.
Journal
Acta Neuropathologica
141(5):697-708. 16 March 2021 (2021)
Details
DOI10.1007/s00401-021-02294-3 PMID33723967 PMCIDPMC8043864 DivisionStructural Studies, Neurobiology - Multi-body Refinement of Cryo-EM Images in RELION AuthorsNakane T., Scheres S. H. W. Journal Methods in Molecular Biology 2215:145-160. January 2021 (2021)
- Tau Protein and Frontotemporal Dementias AuthorsGoedert M., Spillantini M. G., Falcon B., Zhang W., Newell K. L., Hasegawa M., Scheres S. H. W., Ghetti B. Journal Advances in Experimental Medicine and Biology 1281:177-199. January 2021 (2021)
- Exploiting prior knowledge about biological macromolecules in cryo-EM structure determination AuthorsKimanius D., Zickert G., Nakane T., Adler J., Lunz S., Schönlieb C. B., Öktem O., Scheres S. H. W. Journal Iucrj 8(Pt 1):60-75. January 2021 (2021)
- Structures and distributions of SARS-CoV-2 spike proteins on intact virions AuthorsKe Z., Oton J., Qu K., Cortese M., Zila V., McKeane L., Nakane T., Zivanov J., Neufeldt C. J., Cerikan B., Lu J. M., Peukes J., Xiong X., Kräusslich H. G., Scheres S. H. W., Bartenschlager R., Briggs J. A. G. Journal Nature 588(7838):498-502. 17 December 2020 (2020)
- Revealing the structures of megadalton-scale DNA complexes with nucleotide resolution AuthorsKube M., Kohler F., Feigl E., Nagel-Yüksel B., Willner E. M., Funke J. J., Gerling T., Stömmer P., Honemann M. N., Martin T. G., Scheres S. H. W., Dietz H. Journal Nature Communications 11(1):6229. December 2020 (2020)
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Single-particle cryo-EM at atomic resolution
AuthorsNakane T., Kotecha A., Sente A., McMullan G., Masiulis S., Brown P. M. G. E., Grigoras I. T., Malinauskaite L., Malinauskas T., Miehling J., Uchański T., Yu L., Karia D., Pechnikova E. V., de Jong E., Keizer J., Bischoff M., McCormack J., Tiemeijer P., Hardwick S. W., Chirgadze D. Y., Murshudov G., Aricescu A. R., Scheres S. H. W.
Journal
Nature
587(7832):152-156. 5 November 2020 (2020)
Details
DOI10.1038/s41586-020-2829‑0 PMID33087931 PMCIDPMC7611073 DivisionNeurobiology, Structural Studies - Cryo-EM structures of tau filaments AuthorsScheres S. H., Zhang W., Falcon B., Goedert M. Journal Current Opinion in Structural Biology 64:17-25. October 2020 (2020)
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A thermostable, closed SARS-CoV-2 spike protein trimer
AuthorsXiong X., Qu K., Ciazynska K. A., Hosmillo M., Carter A. P., Ebrahimi S., Ke Z., Scheres S. H. W., Bergamaschi L., Grice G. L., Zhang Y.; CITIID-NIHR COVID-19 BioResource Collaboration, Nathan J. A., Baker S., James L. C., Baxendale H. E., Goodfellow I., Doffinger R., Briggs J. A. G.
Journal
Nature Structural & Molecular Biology
27(10):934-941. October 2020 (2020)
Details
DOI10.1038/s41594-020-0478‑5 PMID32737467 PMCIDPMC7116388 DivisionProtein & Nucleic Acid Chemistry, Structural Studies -
Structures of α-synuclein filaments from multiple system atrophy
AuthorsSchweighauser M., Shi Y., Tarutani A., Kametani F., Murzin A. G., Ghetti B., Matsubara T., Tomita T., Ando T., Hasegawa K., Murayama S., Yoshida M., Hasegawa M., Scheres S. H. W., Goedert M.
Journal
Nature
585(7825):464-469. 17 September 2020 (2020)
Details
DOI10.1038/s41586-020-2317-6 PMID32461689 PMCIDPMC7116528 DivisionStructural Studies, Neurobiology -
Novel tau filament fold in corticobasal degeneration
AuthorsZhang W., Tarutani A., Newell K. L., Murzin A. G., Matsubara T., Falcon B., Vidal R., Garringer H. J., Shi Y., Ikeuchi T., Murayama S., Ghetti B., Hasegawa M., Goedert M., Scheres S. H. W.
Journal
Nature
580(7802):283-287. 9 April 2020 (2020)
Details
DOI10.1038/s41586-020-2043‑0 PMID32050258 PMCIDPMC7148158 DivisionNeurobiology, Structural Studies - Cryo-EM structure of a neuronal functional amyloid implicated in memory persistence in Drosophila AuthorsHervas R., Rau M. J., Park Y., Zhang W., Murzin A. G., Fitzpatrick J. A. J., Scheres S. H. W., Si K. Journal Science 367(6483):1230-1234. 13 March 2020 (2020)
- Estimation of high-order aberrations and anisotropic magnification from cryo-EM data sets in RELION-3.1 AuthorsZivanov J., Nakane T., Scheres S. H. W. Journal Iucrj 7(Pt 2):253-267. March 2020 (2020)
- Amyloid structure determination in RELION-3.1 AuthorsScheres S. H. W. Journal Acta crystallographica. Section D, Structural Biology 76(Pt 2):94-101. 1 February 2020 (2020)
- Journal of Structural Biology – Paper of the Year 2019 AuthorsScheres S. H. W. Journal Journal of Structural Biology 209(1):107436. 1 January 2020 (2020)
- Cryo-EM of dynein microtubule-binding domains shows how an axonemal dynein distorts the microtubule AuthorsLacey S. E., He S., Scheres S. H., Carter A. P. Journal ELife 8: e47145. 2 July 2019 (2019)
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Novel tau filament fold in chronic traumatic encephalopathy encloses hydrophobic molecules
AuthorsFalcon B., Zivanov J., Zhang W., Murzin A. G., Garringer H. J., Vidal R., Crowther R. A., Newell K. L., Ghetti B., Goedert M., Scheres S. H. W.
Journal
Nature
568(7752):420-423. April 2019 (2019)
Details
DOI10.1038/s41586-019-1026-5 PMID30894745 PMCIDPMC6472968 DivisionNeurobiology, Structural Studies -
Heparin-induced tau filaments are polymorphic and differ from those in Alzheimer's and Pick's diseases
AuthorsZhang W., Falcon B., Murzin A. G., Fan J., Crowther R. A., Goedert M., Scheres S. H.
Journal
ELife
8: e43584. 5 February 2019 (2019)
Details
DOI10.7554/eLife.43584 PMID30720432 PMCIDPMC6375701 DivisionNeurobiology, Structural Studies - A Bayesian approach to beam-induced motion correction in cryo-EM single-particle analysis AuthorsZivanov J., Nakane T., Scheres S. H. W. Journal Iucrj 6(Pt 1):5-17. January 2019 (2019)
- New tools for automated high-resolution cryo-EM structure determination in RELION-3 AuthorsZivanov J., Nakane T., Forsberg B. O., Kimanius D., Hagen W. J., Lindahl E., Scheres S. H. Journal ELife 7: e42166. 9 November 2018 (2018)
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Tau filaments from multiple cases of sporadic and inherited Alzheimer's disease adopt a common fold
AuthorsFalcon B., Zhang W., Schweighauser M., Murzin A. G., Vidal R., Garringer H. J., Ghetti B., Scheres S. H. W., Goedert M.
Journal
Acta Neuropathologica
136(5):699-708. November 2018 (2018)
Details
DOI10.1007/s00401-018-1914-z PMID30276465 PMCIDPMC6208733 DivisionStructural Studies, Neurobiology -
Structures of filaments from Pick's disease reveal a novel tau protein fold
AuthorsFalcon B., Zhang W., Murzin A. G., Murshudov G., Garringer H. J., Vidal R., Crowther R. A., Ghetti B., Scheres S. H. W., Goedert M.
Journal
Nature
561(7721):137-140. 6 September 2018 (2018)
Details
DOI10.1038/s41586-018-0454-y PMID30158706 PMCIDPMC6204212 DivisionStructural Studies, Neurobiology - Characterisation of molecular motions in cryo-EM single-particle data by multi-body refinement in RELION AuthorsNakane T., Kimanius D., Lindahl E., Scheres S. H. Journal ELife 7: e36861. 1 June 2018 (2018)
- Atomic structure of type VI contractile sheath from Pseudomonas aeruginosa AuthorsSalih O., He S., Planamente S., Stach L., MacDonald J. T., Manoli E., Scheres S. H. W., Filloux A., Freemont P. S. Journal Structure 26(2):329-336.e3. 6 February 2018 (2018)
- Distinct Conformers of Assembled Tau in Alzheimer's and Pick's Diseases AuthorsGoedert M., Falcon B., Zhang W., Ghetti B., Scheres S. H. W. Journal Cold Spring Harbor Symposia on Quantitative Biology 83:163-171. January 2018 (2018)
- The structural basis of proton driven zinc transport by ZntB AuthorsGati C., Stetsenko A., Slotboom D. J., Scheres S. H. W., Guskov A. Journal Nature Communications 8(1):1313. 3 November 2017 (2017)
- CryoEM structures of spliceosomal complexes reveal the molecular mechanism of pre-mRNA splicing AuthorsScheres S. H., Nagai K. Journal Current Opinion in Structural Biology 46:130-139. October 2017 (2017)
- CryoEM structure of MxB reveals a novel oligomerization interface critical for HIV restriction AuthorsAlvarez F. J. D., He S., Perilla J. R., Jang S., Schulten K., Engelman A. N., Scheres S. H. W., Zhang P. Journal Science Advances 3(9):e1701264. 15 September 2017 (2017)
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Cryo-EM structures of tau filaments from Alzheimer's disease
AuthorsFitzpatrick A. W. P., Falcon B., He S., Murzin A. G., Murshudov G., Garringer H. J., Crowther R. A., Ghetti B., Goedert M., Scheres S. H. W.
Journal
Nature
547(7662):185-190 (2017)
Details
DOI10.1038/nature23002 PMID28678775 PMCIDPMC5552202 DivisionNeurobiology, Structural Studies - Helical reconstruction in RELION AuthorsHe S., Scheres S. H. Journal Journal of Structural Biology 198(3):163-176. June 2017 (2017)
- A pipeline approach to single-particle processing in RELION AuthorsFernandez-Leiro R., Scheres S. H. W. Journal Acta Crystallographica. Section D, Structural Biology 73(Pt 6):496-502. 1 June 2017 (2017)
- Structure of the MacAB-TolC ABC-type tripartite multidrug efflux pump AuthorsFitzpatrick A. W. P., Llabrés S., Neuberger A., Blaza J. N., Bai X. C., Okada U., Murakami S., van Veen H. W., Zachariae U., Scheres S. H. W., Luisi B. F., Du D. Journal Nature Microbiology 2:17070. 15 May 2017 (2017)
- Cryo-EM structure of a metazoan separase-securin complex at near-atomic resolution AuthorsBoland A., Martin T. G., Zhang Z., Yang J., Bai X. C., Chang L., Scheres S. H., Barford D. Journal Nature Structural & Molecular Biology 24(4):414-418. April 2017 (2017)
- Mefloquine targets the Plasmodium falciparum 80S ribosome to inhibit protein synthesis AuthorsWong W., Bai X. C., Sleebs B. E., Triglia T., Brown A., Thompson J. K., Jackson K. E., Hanssen E., Marapana D. S., Fernandez I. S., Ralph S. A., Cowman A. F., Scheres S. H., Baum J. Journal Nature Microbiology 2:17031. 13 March 2017 (2017)
- Mechanistic insights into caspase-9 activation by the structure of the apoptosome holoenzyme AuthorsLi Y., Zhou M., Hu Q., Bai X. C., Huang W., Scheres S. H., Shi Y. Journal Proceedings of the National Academy of Sciences of the United States of America 114(7):1542-1547.14 February 2017 (2017)
- Self-correcting mismatches during high-fidelity DNA replication AuthorsFernandez-Leiro R. , Conrad J., Yang J. C., Freund S. M., Scheres S. H., Lamers M. H1. Journal Nature Structural & Molecular Biology 24(2):140-143. February 2017 (2017)
- X-ray and cryo-EM structures of monomeric and filamentous actin-like protein MamK reveal changes associated with polymerization AuthorsLöwe J., He S., Scheres S. H. Savva C. G. Journal Proceedings of the National Academy of Sciences of the United States of America 113(47):13396-13401. 22 November 2016 (2016)
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Design of a molecular support for cryo-EM structure determination
AuthorsMartin T. G., Bharat T. A., Joerger A. C., Bai X. C., Praetorius F., Fersht A. R., Dietz H., Scheres S. H.
Journal
Proceedings of the National Academy of Sciences of the United States of America
113(47):E7456-E7463. 22 November 2016 (2016)
Details
DOI10.1073/pnas.1612720113 PMID27821763 PMCIDPMC5127339 DivisionProtein & Nucleic Acid Chemistry, Structural Studies - Accelerated cryo-EM structure determination with parallelisation using GPUs in RELION-2 AuthorsKimanius D., Forsberg B. O., Scheres S. H., Lindahl E. Journal ELife 5:e18722. 15 November 2016 (2016)
- Resolving macromolecular structures from electron cryo-tomography data using subtomogram averaging in RELION AuthorsBharat T. A., Scheres S. H. Journal Nature Protocols 11(11):2054-2065. November 2016 (2016)
- Unravelling biological macromolecules with cryo-electron microscopy AuthorsFernandez-Leiro R., Scheres S. H. Journal Nature 537(7620):339-346. 15 September 2016 (2016)
- Processing of Structurally Heterogeneous Cryo-EM Data in RELION AuthorsScheres S. H. Journal Methods in Enzymology 579:125-157. 31 May 2016 (2016)
- Cryo-EM structure of the yeast U4/U6. U5 tri-snRNP at 3.7 A resolution AuthorsNguyen T. H., Galej W. P., Bai X. C., Oubridge C., Newman A. J., Scheres S. H., Nagai K. Journal Nature 530(7590):298-302. 18 February 2016 (2016)
- Sampling the conformational space of the catalytic subunit of human γ-secretase AuthorsBai X. C., Rajendra E., Yang G., Shi Y., Scheres S. H. Journal ELife 4: e11182. 1 December 2015 (2015)
- Atomic structure of the apoptosome: mechanism of cytochrome c- and dATP-mediated activation of Apaf-1 AuthorsZhou M., Li Y., Hu Q., Bai X. C., Huang W., Yan C., Scheres S. H., Shi Y. Journal Genes & Development 29(22):2349-2361. 15 November 2015 (2015)
- cryo-EM structures of the E. coli replicative DNA polymerase reveal dynamic interactions with clamp, exonuclease and τ AuthorsFernández-Leiro R., Conrad J., Scheres S. H., Lamers M. H. Journal ELife 4:e11134. 24 October 2015 (2015)
- An atomic structure of human γ-secretase AuthorsBai X. C., Yan C., Yang G., Lu P., Ma D., Sun L., Zhou R., Scheres S. H., Shi Y. Journal Nature 525(7568):212-217. 10 September 2015 (2015)
- Advances in Single-Particle Electron Cryomicroscopy Structure Determination applied to Sub-tomogram Averaging AuthorsBharat T. A., Russo C. J., Löwe J., Passmore L. A., Scheres S. H. Journal Structure 23(9):1743-1753. 1 September 2015 (2015)
- The architecture of the spliceosomal U4/U6. U5 tri‑snRNP AuthorsNguyen T. H., Galej W. P., Bai X. C., Savva C. G., Newman A. J., Scheres S. H., Nagai K. Journal Nature 523(7558):47-52. 2 July 2015 (2015)
- Cryo-EM: A Unique Tool for the Visualization of Macromolecular Complexity AuthorsNogales E., Scheres S. H. Journal Molecular Cell 58(4):677-689. 21 May 2015 (2015)
- Ribosome. The structure of the human mitochondrial ribosome AuthorsAmunts A., Brown A., Toots J., Scheres S. H., Ramakrishnan V. Journal Science 348(6230):95-98. 3 April 2015 (2015)
- Structure of the apoptosome: mechanistic insights into activation of an initiator caspase from Drosophila AuthorsPang Y., Bai X. C., Yan C., Hao Q., Chen Z., Wang J. W., Scheres S. H., Shi Y. Journal Genes & Development 29(3):277-287. 1 February 2015 (2015)
- Semi-automated selection of cryo-EM particles in RELION-1.3. AuthorsScheres S. H. Journal Journal of Structural Biology 189(2):114-122. 1 February 2015 (2015)
- Collaborative Computational Project for Electron cryo‑Microscopy. AuthorsWood C., Burnley T., Patwardhan A., Scheres S., Topf M., Roseman A., Winn M. Journal Acta Crystallographica. Section D, Biological Crystallography 71(Pt 1):123-126, 1 January 2015 (2015)
- How cryo-EM is revolutionizing structural biology. AuthorsBai X. C., McMullan G., Scheres S. H. Journal Trends in Biochemical Sciences 40(1):49-57, 1 January 2015 (2015)
- Structure of the rabbit ryanodine receptor RyR1 at near-atomic resolution. AuthorsYan Z., Bai X. C., Yan C., Wu J., Li Z., Xie T., Peng W., Yin C. C., Li X., Scheres S. H., Shi Y., Yan N. Journal Nature 517(7532):50-55, 1 January 2015 (2015)
- Structure of the large ribosomal subunit from human mitochondria AuthorsBrown A., Amunts A., Bai X. C., Sugimoto Y., Edwards P. C., Murshudov G., Scheres S. H., Ramakrishnan V. Journal Science 346(6210):718-722. 7 November 2014 (2014)
- Three-dimensional structure of human γ-secretase AuthorsLu P., Bai X. C., Ma D., Xie T., Yan C., Sun L., Yang G., Zhao Y., Zhou R., Scheres S. H., Shi Y. Journal Nature 512(7513):166-170. 14 August 2014 (2014)
- Beam-induced motion correction for sub-megadalton cryo-EM particles AuthorsScheres S. H. Journal ELife 3:e03665. 13 August 2014 (2014)
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Structure of the mammalian ribosome-Sec61 complex to 3.4 A resolution
AuthorsVoorhees R. M., Fernández I. S., Scheres S. H., Hegde R. S.
Journal
Cell
157(7):1632-1643. 19 June 2014 (2014)
Details
DOI10.1016/j. Cell.2014.05.024 PMID24930395 PMCIDPMC4081569 DivisionCell Biology, Structural Studies - Cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to the anti-protozoan drug emetine AuthorsWong W., Bai X. C., Brown A., Fernandez I. S., Hanssen E., Condron M., Tan Y. H., Baum J., Scheres S. H. Journal ELife 3:e03080. 9 June 2014 (2014)
- Initiation of translation by cricket paralysis virus IRES requires its translocation in the ribosome AuthorsFernández I. S., Bai X. C., Murshudov G., Scheres S. H., Ramakrishnan V. Journal Cell 157(4):823-831. 8 May 2014 (2014)
- Structure of the yeast mitochondrial large ribosomal subunit AuthorsAmunts A., Brown A., Bai X. C., Llácer J. L., Hussain T., Emsley P., Long F., Murshudov G., Scheres S. H., Ramakrishnan V. Journal Science 343(6178):1485-1489. 28 March 2014 (2014)
- Subunit positioning and stator filament stiffness in regulation and power transmission in the V1 motor of the Manduca sexta V‑ATPase AuthorsMuench S. P., Scheres S. H., Huss M., Phillips C., Vitavska O., Wieczorek H., Trinick J., Harrison M. A. Journal Journal of Molecular Biology 426(2):286-300. 23 January 2014 (2014)
- High-resolution noise substitution to measure overfitting and validate resolution in 3D structure determination by single particle electron cryomicroscopy AuthorsChen S., McMullan G., Faruqi A. R., Murshudov G. N., Short J. M., Scheres S. H., Henderson R. Journal Ultramicroscopy 135C:24-35. December 2013 (2013)
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Molecular architecture of a eukaryotic translational initiation complex
AuthorsFernández I. S., Bai X. C., Hussain T., Kelley A. C., Lorsch J. R., Ramakrishnan V., Scheres S. H.
Journal
Science
342(6160):1240585. 15 November 2013 (2013)
Details
DOI10.1126/science.1240585 PMID24200810 PMCIDPMC3836175 DivisionProtein & Nucleic Acid Chemistry - Structure of active dimeric human telomerase AuthorsSauerwald A., Sandin S., Cristofari G., Scheres S. H., Lingner J., Rhodes D. Journal Nature Structural & Molecular Biology 20(4):454-460. April 2013 (2013)
- Ribosome structures to near-atomic resolution from thirty thousand cryo-EM particles AuthorsBai X. C., Fernandez I. S., McMullan G., Scheres S. H. Journal ELife 2:e00461. 19 February 2013 (2013)
- Cryo-EM structure of a 3D DNA-origami object AuthorsBai X. C., Martin T. G., Scheres S. H., Dietz H. Journal Proceedings of the National Academy of Sciences, USA 109(49):20012-20017. 4 December 2012 (2012)
- RELION: implementation of a Bayesian approach to cryo-EM structure determination AuthorsScheres S. H. Journal Journal of Structural Biology 180(3):519-530. December 2012 (2012)
- Data management challenges in three-dimensional EM AuthorsPatwardhan A., Carazo J. M., Carragher B., Henderson R., Heymann J. B., Hill E., Jensen G. J., Lagerstedt I., Lawson C. L., Ludtke S. J., Mastronarde D., Moore W. J., Roseman A., Rosenthal P., Sorzano C. O., Sanz-García E., Scheres S. H., Subramaniam S., Westbrook J., Winn M., Swedlow J. R., Kleywegt G. J. Journal Nature Structural & Molecular Biology 19(12):1203-1207. December 2012 (2012)
- Prevention of overfitting in cryo-EM structure determination AuthorsScheres S. H., Chen S. Journal Nature Methods 9(9):853-854. September 2012 (2012)
- A Bayesian view on cryo-EM structure determination AuthorsScheres S. H. W. Journal Journal of Molecular Biology 415(2):406-418. 13 January 2012 (2012)
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Electron microscopy studies on the quaternary structure of p53 reveal different binding modes for p53 tetramers in complex with DNA
AuthorsMelero R., Rajagopalan S., Lázaro M., Joerger A. C., Brandt T., Veprintsev D. B., Lasso G., Gil D., Scheres S. H., Carazo J. M., Fersht A. R., Valle M.
Journal
Proceedings of the National Academy of Sciences, USA
108(2):557-562. 11 January 2011 (2011)
Details
DOI10.1073/pnas.1015520107 PMID21178074 PMCIDPMC3021029 DivisionProtein & Nucleic Acid Chemistry, Structural Studies - Classification of structural heterogeneity by maximum-likelihood methods AuthorsScheres S. H. W. Journal Methods in Enzymology 482:295-320. 29 November 2010 (2010)
- Visualizing molecular machines in action: Single-particle analysis with structural variability AuthorsScheres S. H. Journal Advances in Protein Chemistry and Structural Biology 81:89-119. 27 November 2010 (published online) (2010)