Published Research

Found 88 results for Holliger.

• Analysis and control of untemplated DNA polymerase activity for guided synthesis of kilobase-scale DNA sequences AuthorsSimeon D. Castle, Thea C. T. Irvine, Adrian Woolfson, Gregory Linshiz, Blake T. Riley, Ifor D. W. Samuel, Loren Picco, Philipp Holliger, Lauren M. Oldfield, Andrew Hessel, Thomas E. Gorochowski Journal Nature Communications (2026)
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  DOI10.1038/s41467-026-69915-x PMID41748576 PMCID  DivisionProtein & Nucleic Acid Chemistry
• A small polymerase ribozyme that can synthesize itself and its complementary strand AuthorsEdoardo Gianni, Samantha L. Y. Kwok, Christopher J. K. Wan, Kevin Goeji, Bryce E. Clifton, Enrico S. Colizzi, James Attwater, Philipp Holliger Journal Science :eadt2760 (2026)
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  DOI10.1126/science.adt2760 PMID41678588 PMCIDPMC7618777 DivisionProtein & Nucleic Acid Chemistry
• Roles of dimeric intermediates in RNA-catalyzed rolling circle synthesis AuthorsKristofferson E.L., McRae E.K.S., Sørensen N.R., Holliger P., Andersen E.S. Journal Nucleic Acids Research 53(11):gkaf057 (2025)
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  DOI10.1093/nar/gkaf057 PMID40521664 PMCIDPMC12168072 DivisionProtein & Nucleic Acid Chemistry
• Trinucleotide substrates under pH-freeze-thaw cycles enable open-ended exponential RNA replication by a polymerase ribozyme AuthorsAttwater J., Augustin T.L., Curran J.F., Kwok S.L.Y., Ohlendorf L., Gianni E., Holliger P. Journal Nature Chemistry 17(7):1129-1137 (2025)
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  DOI10.1038/s41557-025-01830-y PMID40437192 PMCIDPMC12226338 DivisionProtein & Nucleic Acid Chemistry
• Structural insights into a DNA polymerase reading the xeno nucleic acid HNA AuthorsGutfreund C., Betz K., Abramov M., Coosemans F., Holliger P., Herdewijn P., Marx A. Journal Nucleic Acids Research 53(1):gkae1156 (2025)
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  DOIdoi.org/10.1093/nar/gkae1156 PMID39673482 PMCIDPMC11724289 DivisionProtein & Nucleic Acid Chemistry
• Rapid discovery of high-affinity antibodies via massively parallel sequencing, ribosome display and affinity screening AuthorsPorebski BT, Balmforth M, Browne G, Riley A, Jamali K, Fürst MJLJ, Velic M, Buchanan A, Minter R, Vaughan T, Holliger P. Journal Nature Biomedical Engineering 8(3):214-232 (2024)
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  DOI10.1038/s41551-023-01093‑3 PMID37814006 PMCIDPMC10963267 DivisionProtein & Nucleic Acid Chemistry, Structural Studies
• Cryo-EM structure and functional landscape of an RNA polymerase ribozyme AuthorsMcRae E. K. S., Wan C. J . K., Kristoffersen E. L., Hansen K., Gianni E., Gallego I., Curran J. F., Attwater J., Holliger P., Andersen E. S. Journal Proceedings of the National Academy of Sciences of the United States of America 21(3):e2313332121 (2024)
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  DOI10.1073/pnas.2313332121 PMID38207080 PMCIDPMC10801858 DivisionProtein & Nucleic Acid Chemistry
• Characterization of an HNA aptamer suggests a non-canonical G-quadruplex motif AuthorsSchofield P., Taylor A. I., Rihon J., Peña Martinez C. D., Zinn S., Mattelaer C. A., Jackson J., Dhaliwal G., Schepers G., Herdewijn P., Lescrinier E., Christ D., Holliger P. Journal Nucleic Acids Research 51(15):7736-7748 (2023)
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  DOI10.1093/nar/gkad592 PMID37439359 PMCIDPMC10450178 DivisionProtein & Nucleic Acid Chemistry
• Emergence of ATP- and GTP-Binding Aptamers from Single RNA Sequences by Error-Prone Replication and Selection AuthorsDr. Falk Wachowius, Dr. Benjamin T. Porebski, Dr. Christopher M. Johnson, Dr. Philipp Holliger Journal Chemsystemschem 5(5):syst.202300006 (2023)
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  DOI10.1002/syst.202300006 PMID38074198 PMCIDPMC7615363 DivisionProtein & Nucleic Acid Chemistry
• The Expanded Central Dogma: Genome Resynthesis, Orthogonal Biosystems, Synthetic Genetics AuthorsGerecht K., Freund N., Liu W., Liu Y., Fürst M. J. L. J., Holliger P. Journal Annual Review of Biophysics 52:413-432 (2023)
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  DOI10.1146/annurev-biophys-111622‑091203 PMID37159296 PMCIDPMC7614659 DivisionProtein & Nucleic Acid Chemistry
• A two-residue nascent-strand steric gate controls synthesis of 2'-O-methyl- and 2'-O-(2-methoxyethyl)-RNA AuthorsFreund N., Taylor A.I., Arangundy-Franklin S., Subramanian N., Peak-Chew S.Y., Whitaker A.M., Freudenthal B.D., Abramov M., Herdewijn P., Holliger P. Journal Nature Chemistry 15(1):91-100 (2022)
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  DOI10.1038/s41557-022-01050‑8 PMID36229679 PMCIDPMC7614059 DivisionProtein & Nucleic Acid Chemistry
• Introduction to the themed collection on XNA xeno-nucleic acids AuthorsBong D, Holliger P, Yang C. Journal RSC Chemical Biology Oct 11;3(11):1299-1300 (2022)
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  DOI10.1039/d2cb90036j PMID36349221 PMCIDPMC9627736 DivisionProtein & Nucleic Acid Chemistry
• A modular XNAzyme cleaves long, structured RNAs under physiological conditions and enables allele-specific gene silencing AuthorsTaylor A.I., Wan C.J.K., Donde M.J., Peak-Chew S.Y., Holliger P. Journal Nature Chemistry 14(11):1295-1305 (2022)
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  DOI10.1038/s41557-022-01021‑z PMID36064973 PMCIDPMC7613789 DivisionProtein & Nucleic Acid Chemistry; Cell Biology
• Efficient synthesis and replication of diverse sequence libraries composed of biostable nucleic acid analogues AuthorsHervey JRD, Freund N, Houlihan G, Dhaliwal G, Holliger P, Taylor AI. Journal RSC Chemical Biology Aug 30;3(10):1209-1215 (2022)
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  DOI10.1039/d2cb00035k PMID36320888 PMCIDPMC9533476 DivisionProtein & Nucleic Acid Chemistry
• On gene silencing by the X10-23 DNAzyme AuthorsTaylor A.I., Holliger P. Journal Nature Chemistry 14(8):855-858 (2022)
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  DOI10.1038/s41557-022-00990-5 PMID35851839 PMCID  DivisionProtein & Nucleic Acid Chemistry
• Hydrophobic-cationic peptides modulate RNA polymerase ribozyme activity by accretion AuthorsLi P., Holliger P., Tagami S. Journal Nature Communications 13(1):3050 (2022)
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  DOI10.1038/s41467-022-30590‑3 PMID35665749 PMCIDPMC9166800 DivisionProtein & Nucleic Acid Chemistry
• New chemistries and enzymes for synthetic genetics AuthorsFreund N., Fürst M. J. L. J., Holliger P. Journal Current Opinion in Biotechnology 74:129-136 (2022)
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  DOI10.1016/j. Copbio.2021.11.004 PMID34883451 PMCID  DivisionProtein & Nucleic Acid Chemistry
• Rolling circle RNA synthesis catalysed by RNA AuthorsKristoffersen E. L., Burman M., Noy A., Holliger P. Journal Elife 11:e75186 (2022)
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  DOI10.7554/elife.75186 PMID35108196 PMCIDPMC8937235 DivisionProtein & Nucleic Acid Chemistry
• Direct Mapping of Higher-Order RNA Interactions by SHAPE‑JuMP AuthorsChristy T. W., Giannetti C. A., Houlihan G., Smola M. J., Rice G. M., Wang J., Dokholyan N. V., Laederach A., Holliger P., Weeks K. M. Journal Biochemistry 60(25):1971-1982. 29 June 2021 (2021)
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  DOI10.1021/acs. Biochem.1c00270 PMID34121404 PMCID  DivisionProtein & Nucleic Acid Chemistry
• Structural Studies of HNA Substrate Specificity in Mutants of an Archaeal DNA Polymerase Obtained by Directed Evolution AuthorsSamson C., Legrand P., Tekpinar M., Rozenski J., Abramov M., Holliger P., Pinheiro V. B., Herdwijn P., Delarue M. Journal Biomolecules 10(12):1647. 8 December 2020 (2020)
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  DOI10.3390/biom10121647 PMID33302546 PMCIDPMC7763228 DivisionProtein & Nucleic Acid Chemistry
• Modified nucleic acids: replication, evolution, and next-generation therapeutics AuthorsDuffy K., Arangundy-Franklin S., Holliger P. Journal BMC Biology 18(1):112. 2 September 2020 (2020)
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  DOI10.1186/s12915-020-00803‑6 PMID32878624 PMCIDPMC7469316 DivisionProtein & Nucleic Acid Chemistry
• Discovery and evolution of RNA and XNA reverse transcriptase function and fidelity AuthorsHoulihan G., Arangundy-Franklin S., Porebski B. T., Subramanian N., Taylor A. I., Holliger P. Journal Nature Chemistry 12(8):683-690. August 2020 (2020)
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  DOI10.1038/s41557-020-0502‑8 PMID32690899 PMCIDPMC7116287 DivisionProtein & Nucleic Acid Chemistry
• Selection of 2'-Deoxy-2'-Fluoroarabino Nucleic Acid (FANA) Aptamers That Bind HIV-1 Integrase with Picomolar Affinity AuthorsRose K. M., Alves Ferreira-Bravo I., Li M., Craigie R., Ditzler M. A., Holliger P., DeStefano J. J. Journal ACS Chemical Biology 14(10):2166-2175. 18 October 2019 (2019)
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  DOI10.1021/acschembio.9b00237 PMID31560515 PMCIDPMC7005942 DivisionProtein & Nucleic Acid Chemistry
• Non-Enzymatic Assembly of a Minimized RNA Polymerase Ribozyme AuthorsWachowius F., Holliger P. Journal Chemsystemschem 1(1-2):1-4 (2019)
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  DOI10.1002/syst.201900004 PMID31673682 PMCIDPMC6823052 DivisionProtein & Nucleic Acid Chemistry
• Beyond DNA and RNA: The Expanding Toolbox of Synthetic Genetics AuthorsTaylor A. I., Houlihan G., Holliger P. Journal Cold Spring Harbor Perspectives in Biology 11(6). June 2019 (2019)
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  DOI10.1101/cshperspect. A032490 PMID31160351 PMCIDPMC6546049 DivisionProtein & Nucleic Acid Chemistry
• A synthetic genetic polymer with an uncharged backbone chemistry based on alkyl phosphonate nucleic acids AuthorsArangundy-Franklin S., Taylor A. I., Porebski B. T., Genna V., Peak-Chew S., Vaisman A., Woodgate R., Orozco M., Holliger P. Journal Nature Chemistry 11(6):533-542. 22 April 2019 (2019)
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  DOI10.1038/s41557-019-0255‑4 PMID31011171 PMCIDPMC6542681 DivisionProtein & Nucleic Acid Chemistry
• Random-sequence genetic oligomer pools display an innate potential for ligation and recombination AuthorsMutschler H., Taylor A. I., Porebski B. T., Lightowlers A., Houlihan G., Abramov M., Herdewijn P., Holliger P. Journal ELife 7:e43022. 21 November 2018 (2018)
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  DOI10.7554/eLife.43022 PMID30461419 PMCIDPMC6289569 DivisionProtein & Nucleic Acid Chemistry
• Selecting Fully-Modified XNA Aptamers Using Synthetic Genetics AuthorsTaylor A. I., Holliger P. Journal Current Protocols in Chemical Biology 10(2):e44. June 2018 (2018)
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  DOI10.1002/cpch.44 PMID29927117 PMCID  DivisionProtein & Nucleic Acid Chemistry
• Ribozyme-catalysed RNA synthesis using triplet building blocks AuthorsAttwater J., Raguram A., Morgunov A. S., Gianni E., Holliger P. Journal ELife 7: e35255. 15 May 2018 (2018)
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  DOI10.7554/eLife.35255 PMID29759114 PMCIDPMC6003772 DivisionProtein & Nucleic Acid Chemistry
• Engineering and application of polymerases for synthetic genetics AuthorsHoulihan G., Arangundy-Franklin S., Holliger P. Journal Current Opinion in Biotechnology 48:168-179. December 2017 (2017)
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  DOI10.1016/j. Copbio.2017.04.004 PMID28601700 PMCID  DivisionProtein & Nucleic Acid Chemistry
• Exploring the Chemistry of Genetic Information Storage and Propagation through Polymerase Engineering AuthorsHoulihan G., Arangundy-Franklin S., Holliger P. Journal Accounts of Chemical Research 50(4):1079-1087. 18 April 2017 (2017)
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  DOI10.1021/acs. Accounts.7b00056 PMID28383245 PMCIDPMC5406124 DivisionProtein & Nucleic Acid Chemistry
• Simple peptides derived from the ribosomal core potentiate RNA polymerase ribozyme function AuthorsTagami S., Attwater J., Holliger P. Journal Nature Chemistry 9(4):325-332. April 2017 (2017)
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  DOI10.1038/nchem.2739 PMID28338682 PMCIDPMC5458135 DivisionProtein & Nucleic Acid Chemistry
• Nucleic acids: function and potential for abiogenesis AuthorsWachowius F., Attwater J., Holliger P. Journal Quarterly Reviews of Biophysics 50:e4. 9 March 2017 (2017)
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  DOI10.1017/S0033583517000038 PMID29233216 PMCID  DivisionProtein & Nucleic Acid Chemistry
• Nanostructures from Synthetic Genetic Polymers AuthorsTaylor A. I., Beuron F., Peak-Chew S. Y., Morris E. P., Herdewijn P., Holliger P. Journal Chembiochem 17(12):1107-1110. 16 June 2016 (2016)
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  DOI10.1002/cbic.201600136 PMID26992063 PMCIDPMC4973672 DivisionProtein & Nucleic Acid Chemistry
• A polymerase engineered for bisulfite sequencing AuthorsMillar D., Christova Y., Holliger P. Journal Nucleic Acids Research 43(22):e155. 15 December 2015 (2015)
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  DOI10.1093/nar/gkv798 PMID26271989 PMCIDPMC4678845 DivisionProtein & Nucleic Acid Chemistry
• Enzymatic Synthesis of Nucleic Acids with Defined Regioisomeric 2'-5' Linkages AuthorsCozens C., Mutschler H., Nelson G. M, Houlihan G., Taylor A. I., Holliger P. Journal Angewandte Chemie 54(51): 15570-15573. 14 December 2015 (2015)
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  DOI10.1002/anie.201508678 PMID26527364 PMCIDPMC4736440 DivisionProtein & Nucleic Acid Chemistry
• Selection of 2'-deoxy-2'-fluoroarabinonucleotide (FANA) aptamers that bind HIV-1 reverse transcriptase with picomolar affinity AuthorsAlves Ferreira-Bravo I., Cozens C., Holliger P., DeStefano J. J. Journal Nucleic Acids Research 43(20):9587-9599. 16 November 2015 (2015)
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  DOI10.1093/nar/gkv1057 PMID26476448 PMCIDPMC4751925 DivisionProtein & Nucleic Acid Chemistry
• Directed evolution of artificial enzymes (XNAzymes) from diverse repertoires of synthetic genetic polymers AuthorsTaylor A. I., Holliger P. Journal Nature Protocols 10(10):1625-1642. October 2015 (2015)
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  DOI10.1038/nprot.2015.104 PMID26401917 PMCID  DivisionProtein & Nucleic Acid Chemistry
• Freeze-thaw cycles as drivers of complex ribozyme assembly AuthorsMutschler H., Wochner A., Holliger P. Journal Nature Chemistry 7(6):502-508. June 2015 (2015)
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  DOI10.1038/nchem.2251 PMID25991529 PMCIDPMC4495579 DivisionProtein & Nucleic Acid Chemistry
• Isoguanine and 5-Methyl-Isocytosine Bases, In Vitro and In Vivo. AuthorsBande O., Abu El Asrar R., Braddick D., Dumbre S., Pezo V., Schepers G., Pinheiro V. B., Lescrinier E., Holliger P., Marlière P., Herdewijn P. Journal Chemistry (Weinheim an der Bergstrasse, Germany) 21(13):5009-5022: 23 March 2015 (2015)
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  DOI10.1002/chem.201406392 PMID25684598 PMCIDPMC4531829 DivisionProtein & Nucleic Acid Chemistry
• Catalysts from synthetic genetic polymers. AuthorsTaylor A. I., Pinheiro V. B., Smola M. J., Morgunov A. S., Peak-Chew S., Cozens C., Weeks K. M., Herdewijn P., Holliger P. Journal Nature 518(7539):427-430. 19 February 2015 (2015)
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  DOI10.1038/nature13982 PMID25470036 PMCIDPMC4336857 DivisionProtein & Nucleic Acid Chemistry
• Subunit disassembly and inhibition of TNFα by a semi-synthetic bicyclic peptide. AuthorsLuzi S., , Bernard E., Stadler L. K., Vaysburd M., Winter G., Holliger P. Journal Protein Engineering, Design & Selection : PEDS 28(2):45-52. 1 February 2015 (2015)
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  DOI10.1093/protein/gzu055 PMID25614525 PMCIDPMC4378371 DivisionProtein & Nucleic Acid Chemistry
• Towards applications of synthetic genetic polymers in diagnosis and therapy AuthorsTaylor A. I., Arangundy-Franklin S., Holliger P. Journal Current Opinion in Chemical Biology 22:79-84. October 2014 (2014)
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  DOI10.1016/j. Cbpa.2014.09.022 PMID25285754 PMCID  DivisionProtein & Nucleic Acid Chemistry
• Towards XNA nanotechnology: new materials from synthetic genetic polymers AuthorsPinheiro V. B., Holliger P. Journal Trends in Biotechnology 32(6):321-328. June 2014 (2014)
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  DOI10.1016/j.tibtech.2014.03.010 PMID24745974 PMCIDPMC4039137 DivisionProtein & Nucleic Acid Chemistry
• A synthetic approach to abiogenesis AuthorsAttwater J., Holliger P. Journal Nature Methods 11(5):495-498. May 2014 (2014)
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  DOI10.1038/nmeth.2893 PMID24781322 PMCID  DivisionProtein & Nucleic Acid Chemistry
• Non-canonical 3'-5' extension of RNA with prebiotically plausible ribonucleoside 2',3'-cyclic phosphates AuthorsMutschler H., Holliger P. Journal Journal of the American Chemical Society 136(14):5193-5196. 9 April 2014 (2014)
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  DOI10.1021/ja4127714 PMID24660752 PMCIDPMC4333585 DivisionProtein & Nucleic Acid Chemistry
• In-ice evolution of RNA polymerase ribozyme activity AuthorsAttwater J., Wochner A., Holliger P. Journal Nature Chemistry 5(12):1011-1018. December 2013 (2013)
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  DOI10.1038/nchem.1781 PMID24256864 PMCIDPMC3920166 DivisionProtein & Nucleic Acid Chemistry
• Structures of an apo and a binary complex of an evolved archeal B family DNA polymerase capable of synthesising highly cy-dye labelled DNA AuthorsWynne S. A., Pinheiro V. B., Holliger P., Leslie A. G. Journal PloS one 8(8):e70892. 5 August 2013 (2013)
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  DOI10.1371/journal.pone.0070892 PMID23940661 PMCIDPMC3733885 DivisionProtein & Nucleic Acid Chemistry, Structural Studies
• Synthetic polymers and their potential as genetic materials AuthorsPinheiro V. B., Loakes D., Holliger P. Journal BioEssays 35(2):113-122. February 2013 (2013)
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  DOI10.1002/bies.201200135 PMID23281109 PMCID  DivisionProtein & Nucleic Acid Chemistry
• Origins of life: The cooperative gene AuthorsAttwater J., Holliger P. Journal Nature 491(7422):48-49. 1 November 2012 (2012)
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  DOI10.1038/nature11635 PMID23075847 PMCID  DivisionProtein & Nucleic Acid Chemistry
• Reversible Fluorescence Photoswitching in DNA AuthorsSmith D. A., Holliger P., Flors C. Journal Journal of Physical Chemistry. B 116(34):10290–10293. 30 August 2012 (2012)
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  DOI10.1021/jp3056834 PMID22861666 PMCIDPMC3439957 DivisionProtein & Nucleic Acid Chemistry
• The XNA world: progress towards replication and evolution of synthetic genetic polymers AuthorsPinheiro V. B., Holliger P. Journal Current Opinion in Chemical Biology 16(3-4):245-252. August 2012 (2012)
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  DOI10.1016/j. Cbpa.2012.05.198 PMID22704981 PMCID  DivisionProtein & Nucleic Acid Chemistry
• A short adaptive path from DNA to RNA polymerases AuthorsCozens C., Pinheiro V. B., Vaisman A., Woodgate R., Holliger P. Journal Proceedings of the National Academy of Sciences, USA 109(21):8067-8072. 22 May 2012 (2012)
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  DOI10.1073/pnas.1120964109 PMID22566643 PMCIDPMC3361454 DivisionProtein & Nucleic Acid Chemistry
• Synthetic genetic polymers capable of heredity and evolution AuthorsPinheiro V. B., Taylor A. I., Cozens C., Abramov M., Renders M., Zhang S., Chaput J. C., Wengel J., Peak-Chew S. Y., McLaughlin S. H., Herdewijn P., Holliger P. Journal Science 336(6079):341-344. 20 April 2012 (2012)
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  DOI10.1126/science.1217622 PMID22517858 PMCIDPMC3362463 DivisionProtein & Nucleic Acid Chemistry; Cell Biology
• Ribozyme-catalyzed transcription of an active ribozyme AuthorsWochner A., Attwater J., Coulson A., Holliger P. Journal Science 332(6026):209-212. 8 April 2011 (2011)
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  DOI10.1126/science.1200752 PMID21474753 PMCIDPMC3069738 DivisionProtein & Nucleic Acid Chemistry
• Molecular breeding of polymerases for resistance to environmental inhibitors AuthorsBaar C., d'Abbadie M., Vaisman A., Arana M. E., Hofreiter M., Woodgate R., Kunkel T A., Holliger P. Journal Nucleic Acids Research 39(8):e51. April 2011 (2011)
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  DOI10.1093/nar/gkq1360 PMID21297114 PMCIDPMC3082909 DivisionProtein & Nucleic Acid Chemistry
• Chemical biotechnology-a marriage of convenience and necessity AuthorsJaeger K. E., Holliger P. Journal Current Opinion in Biotechnology 21(6):711-712. 1 December 2010 (2010)
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  DOI10.1016/j. Copbio.2010.09.017 PMID20971630 PMCID  DivisionProtein & Nucleic Acid Chemistry
• Ice as a protocellular medium for RNA replication AuthorsAttwater J., Wochner A., Pinheiro V. B., Coulson A., Holliger P. Journal Nature Communications 1:76. 21 September 2010 (2010)
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  DOIdoi:10.1038/ncomms1076 PMID20865803 PMCID  DivisionProtein & Nucleic Acid Chemistry
• CyDNA: synthesis and replication of highly Cy-dye substituted DNA by an evolved polymerase AuthorsRamsay N., Jemth A. S., Brown A., Crampton N., Dear P., Holliger P. Journal Journal of the American Chemical Society 132(14):5096-5104. 14 April 2010 (2010)
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  DOI0.1021/ja909180c PMID20235594 PMCIDPMC2850551 DivisionProtein & Nucleic Acid Chemistry
• Evolving a polymerase for hydrophobic base analogues AuthorsLoakes D., Gallego J., Pinheiro V. B., Kool E. T., Holliger P. Journal Journal of the American Chemical Society 131(41):14827-14837. 24 September 2009 (2009)
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  DOI10.1021/ja9039696 PMID19778048 PMCIDPMC2762193 DivisionProtein & Nucleic Acid Chemistry
• Darwinian chemistry: towards the synthesis of a simple cell AuthorsDavid Loakes, Philipp Holliger Journal Molecular BioSystems 5(7):686-694 (2009) (2009)
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  DOI10.1039/b904024b PMID19562107 PMCID  DivisionProtein & Nucleic Acid Chemistry
• Polymerase engineering: towards the encoded synthesis of unnatural biopolymers AuthorsDavid Loakes, Philipp Holliger Journal Chemical Communications (31):4619-4631 (2009) (2009)
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  DOI10.1039/b903307f PMID19641798 PMCID  DivisionProtein & Nucleic Acid Chemistry
• Translesion synthesis across the (6-4) photoproduct and its Dewar valence isomer by the Y-family and engineered DNA polymerases AuthorsYamamoto J., Loakes D., Masutani C., Simmyo S., Urabe K., Hanaoka F., Holliger P., Iwai S. Journal Nucleic Acids Symposium Series (52):339-340. January 2008 (2008)
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  DOI10.1093/nass/nrn171 PMID18776392 PMCID  DivisionProtein & Nucleic Acid Chemistry
• Molecular breeding of polymerases for amplification of ancient DNA AuthorsMarc d'Abbadie, Hofreiter M., Vaisman A., Loakes D., Gasparutto D., Cadet J., Woodgate R., Paabo S., Holliger P. Journal Nature Biotechnology 25(8): 939-943 (2007) (2007)
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  DOI10.1038/nbt1321 PMID17632524 PMCIDPMC1978225 Division 
• Compartmentalized self-replication: a novel method for the directed evolution of polymerases and other enzymes AuthorsF. J. Ghadessy, P. Holliger Journal Methods Mol. Biol. 352: 237-248 (2007) (2007)
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  DOI10.1385/1-59745-187-8:237 PMID17041269 PMCID  Division 
• Inhibition of tumor growth in vivo by in situ secretion of bispecific anti-CEA x anti-CD3 diabodies from lentivirally transduced human lymphocytes AuthorsM. Compte, B. Blanco, F. Serrano, A. M. Cuesta, L. Sanz, A. Bernad, P. Holliger, L. Alvarez‑Vallina Journal Cancer Gene Therapy 14: 380-388 (2007) (2007)
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  DOI  PMID  PMCID  Division 
• Directed evolution of DNA polymerase, RNA polymerase and reverse transcriptase activity in a single polypeptide AuthorsJennifer L. Ong, David Loakes, Szymon Jaroslawski, Kathleen Too, Philipp Holliger Journal J. Mol. Biol. 361: 537-550 (2006) (2006)
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  DOI  PMID  PMCID  Division 
• Effect of a hydrogen bonding carboxamide group on universal bases AuthorsKathleen Too, Daniel M. Brown, Philipp Holliger, David Loakes Journal Collect. Czech. Chem. Commun. 71(6): 899-911 (2006) (2006)
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  DOI  PMID  PMCID  Division 
• Engineered antibody fragments and the rise of single domains AuthorsHolliger P., Hudson P. J. Journal Nature Biotechnology 23(9):1126-1136. September 2005 (2005)
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  DOI10.1038/nbt1142 PMID16151406 PMCID  DivisionProtein & Nucleic Acid Chemistry
• Engineering high affinity superantigens by phage display AuthorsCarolyn Enever, Ian M. Tomlinson, John Lund, Michaela Levens, Philipp Holliger Journal Journal of Molecular Biology 347: 107-120 (2005) (2005)
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  DOI  PMID  PMCID  Division 
• Generic expansion of the substrate spectrum of a DNA polymerase by directed evolution AuthorsFarid J. Ghadessy, Nicola Ramsay, Francois Boudsocq, David Loakes, Anthony Brown, Shigenori Iwai, Alexandra Vaisman, Roger Woodgate, Philipp Holliger Journal Nature Biotechnology 22: 755-759 (2004) (2004)
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  DOI  PMID  PMCID  Division 
• A novel emulsion mixture for in vitro compartmentalization of transcription and translation in the rabbit reticulocyte system AuthorsFarid J. Ghadessy, Philipp Holliger Journal Protein Engineering, Design & Selection 17: 201-204 (2004) (2004)
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  DOI  PMID  PMCID  Division 
• Induction of human T lymphocyte cytotoxicity and inhibition of tumor growth by tumor-specific diabody-based molecules secreted from gene-modified bystander cells AuthorsBelen Blanco, Philipp Holliger, Richard G. Vile, Luis Alvarez‑Vallina Journal Journal of Immunology 171: 1070-1077 (2003) (2003)
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  DOI  PMID  PMCID  Division 
• Isolation of receptor-ligand pairs by capture of long-lived multivalent interaction complexes AuthorsRuud M. T. de Wildt, Ian M. Tomlinson, Jennifer L. Ong, Philipp Holliger Journal Proceedings of the National Academy of Sciences, USA 99: 8530-8535 (2002) (2002)
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  DOI  PMID  PMCID  Division 
• Directed evolution of polymerase function by compartmentalized self‑replication AuthorsFarid J. Ghadessy, Jennifer L. Ong, Philipp Holliger Journal Proceedings of the National Academy of Sciences, USA 98(8):4552-4557. 10 April 2001 (2001)
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  DOI10.1073/pnas.071052198 PMID11274352 PMCIDPMC31872 DivisionProtein & Nucleic Acid Chemistry
• Crystal structure of the two N-terminal domains of g3p from filamentous phage fd at 1.9 Angstrom: evidence for conformational lability AuthorsHolliger P., Riechmann L., Williams R. L. Journal Journal of Molecular Biology 288(4):649-657. 14 May 1999 (1999)
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  DOI10.1006/jmbi.1999.2720 PMID10329170 PMCID  DivisionProtein & Nucleic Acid Chemistry
• Carcinoembryonic antigen (CEA)-specific T-cell activation in colon carcinoma induced by anti-CD3xAnti-CEA bispecific diabodies and B7xAnti-CEA bispecific fusion proteins AuthorsPhilipp Holliger, Oliver Manzke, Mary Span, Robert Hawkins, Bernd Fleischmann, Liu Qinghua, Jurgen Wolf, Volker Diehl, Olivier Cochet, Greg Winter, Heribert Bohlen Journal Cancer Reseach 59: 2909-2916 (1999) (1999)
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  DOI  PMID  PMCID  Division 
• Diabodies : small bispecific antibody fragments AuthorsHolliger P., Winter G. Journal Cancer Immunology and Immunotherapy 45(3-4):128-130. Nov-Dec 1997 (1997)
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  DOI10.1007/s002620050414 PMID9435855 PMCID  DivisionProtein & Nucleic Acid Chemistry
• Improved tumour targeting by disulphide stabilized diabodies expressed in Pichia pastoris AuthorsFitzGerald K., Holliger P., Winter G. Journal Protein Engineering 10(10):1221-1225. October 1997 (1997)
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  DOI10.1093/protein/10.10.1221 PMID9488147 PMCID  DivisionProtein & Nucleic Acid Chemistry
• Retargeting serum immunoglobulin with bispecific diabodies AuthorsHolliger P., Wing M., Pound J. D., Bohlen H., Winter G. Journal Nature Biotechnology 15(7):632-636. July 1997 (1997)
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  DOI10.1038/nbt0797‑632 PMID9219264 PMCID  DivisionProtein & Nucleic Acid Chemistry
• The 2.0-Angstrom resolution crystal strucutre of a trimeric antibody fragment with noncognate VH-VL domain pairs shows a rearrangement of VH CDR3 AuthorsXue Y. Pei, Philipp Holliger, Alexey G. Murzin, Roger L. Williams Journal Proceedings of the National Academy of Sciences, USA 94: 9637-9642 (1997) (1997)
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• The C-terminal domain of TolA is the coreceptor for filamentous phage infection of E. coli AuthorsLutz Riechmann, Philipp Holliger Journal Cell 90: 351-360 (1997) (1997)
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• Mimicking somatic hypermutation: affinity maturation of antibodies displayed on bacteriophage using a bacterial mutator strain AuthorsNigel M. Low, Philipp Holliger, Greg Winter Journal Journal of Molecular Biology 260: 359-368 (1996) (1996)
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• Specific killing of lymphoma cells by cytoxic T-cells mediated by a bispecific diabody AuthorsPhilipp Holliger, Jan Brissinck, Roger L. Williams, Kris Thielemans, Greg Winter Journal Protein Engineering 9 : 299-305 (1996) (1996)
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• Crystal structure of a diabody, a bivalent antibody fragment AuthorsOlga Perisic, Philip A. Webb, Philipp Holliger, Greg Winter, Roger L. Williams Journal Structure 2: 1217-1226 (1994) (1994)
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• "Diabodies": small bivalent and bispecific antibody fragments AuthorsPhilipp Holliger, Terence Prospero, Greg Winter Journal Proceedings of the National Academy of Sciences, USA 90: 6444-6448 (1993) (1993)
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• Human anti-self antibodies with high specificity from phage display libraries AuthorsAndrew D. Griffiths, Magnus Malmqvist, James D. Marks, Jacqueline M. Bye, M. J. Embleton, John McCafferty, Michael Baier, K. Philipp Holliger, Barbara D. Gorick, Nevin C. Hughes-Jones, Hennie R. Hoogenboom, Greg Winter Journal EMBO Journal 12: 725-734 (1993) (1993)
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• Engineering bispecific antibodies AuthorsPhilipp Holliger, Greg Winter Journal Current Opinion in Biotechnology 4: 446-449 (1993) (1993)
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