Published Research
Found 47 results for Barford.
- Mechanisms of ubiquitylation of the mitotic regulatory protein Cdc20 AuthorsSitry-Shevah D., Miniowitz-Shemtov S., Liburkin Dan T., Barford D., Hershko A. Journal Proceedings of the National Academy of Sciences of the United States of America 123(24):e2612003123 (2026)
- Models for the architecture of the human inner kinetochore on centromeric α-satellite CENP-A nucleosome arrays AuthorsYu C., Muir K. W., Yang J., Zhang Z., McLaughlin S. H., Barford D. Journal Nature Communications 17(1):6346 (2026)
- Assembly and phosphoregulatory mechanisms of the budding yeast outer kinetochore KMN complex AuthorsTurner N. N., Zhang Z., Yang J., Muir K. W., McLaughlin S. H., Morgan T., Barford D. Journal The Journal of Cell Biology 225(5):e202506015 (2026)
- Development of D-box peptides to inhibit the anaphase-promoting complex/cyclosome AuthorsEapen R., Okoye C., Stubbs C., Schimpl M., Tischer T., Fisher E.J., Zacharopoulou M., Ferrer F., Barford D., Spring D.R., Lindon C., Phillips C., Itzhaki L.S. Journal Elife 14 (2025)
- Molecular mechanism of Mad2 conformational conversion promoted by the Mad2-interaction motif of Cdc20 AuthorsYu C.W.H., Fischer E.S., Greener J.G., Yang J., Zhang Z., Freund S.M.V., Barford D. Journal Protein Science : a Publication of the Protein Society 34(4):e70099 (2025)
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Cryo-EM structures of apo-APC/C and APC/C(CDH1:EMI1) complexes provide insights into APC/C regulation
AuthorsHöfler A., Yu J., Yang J., Zhang Z., Chang L., McLaughlin S. H., Grime G. W., Garman E. F., Boland A., Barford D.
Journal
Nature Communications
15(1):10074 (2024)
Details
DOIdoi.org/10.1038/s41467-024-54398-5 PMID39567505 PMCIDPMC11579458 DivisionStructural Studies, Facilities (Biophys) - A comparative study of the cryo-EM structures of Saccharomyces cerevisiae and human anaphase-promoting complex/cyclosome (APC/C) AuthorsVazquez-Fernandez E., Yang J., Zhang Z., Andreeva A. E., Emsley P., Barford D. Journal Elife 13:RP100821 (2024)
- Structure of the human outer kinetochore KMN network complex AuthorsYatskevich S, Yang J, Bellini D, Zhang Z, Barford D. Journal Nature Structural & Molecular Biology 31(6):874-883 (2024)
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Structure of the native γ-tubulin ring complex capping spindle microtubules
AuthorsDendooven T, Yatskevich S, Burt A, Chen ZA, Bellini D, Rappsilber J, Kilmartin JV, Barford D.
Journal
Nature Structural & Molecular Biology
31(7):1134-1144 (2024)
Details
DOI10.1038/s41594-024-01281‑y PMID38609662 PMCIDPMC11257966 DivisionStructural Studies, Cell Biology -
Structural mechanism of outer kinetochore Dam1-Ndc80 complex assembly on microtubules
AuthorsMuir K.W., Batters C., Dendooven T., Yang J., Zhang Z., Burt A., Barford D.
Journal
Science
382(6675):1184-1190. (2023)
Details
DOIdoi.org/10.1126/science.adj8736 PMID38060647 PMCIDPMC7615550 DivisionStructural Studies Facilities - Biophysics - Cryo-EM structure of the complete inner kinetochore of the budding yeast point centromere AuthorsDendooven T., Zhang Z., Yang J., McLaughlin S. H., Schwab J., Scheres S. H. W., Yatskevich S., Barford D. Journal Science Advances 9(30):eadg7480 (2023)
- Conserved and divergent mechanisms of inner kinetochore assembly onto centromeric chromatin AuthorsYatskevich S., Barford D., Muir K. W. Journal Current Opinion in Structural Biology 81:102638 (2023)
- Biasing the conformation of ELMO2 reveals that myoblast fusion can be exploited to improve muscle regeneration AuthorsTran V, Nahlé S, Robert A, Desanlis I, Killoran R, Ehresmann S, Thibault MP, Barford D, Ravichandran KS, Sauvageau M, Smith MJ, Kmita M, Côté JF. Journal Nature Communications Nov 18;13(1):7077 (2022)
- Structural Biology of DOCK-Family Guanine Nucleotide Exchange Factors AuthorsBoland A., Côté J. F, Barford D. Journal FEBS Letters 597(6):794-810 (2023)
- Juxtaposition of Bub1 and Cdc20 on phosphorylated Mad1 during catalytic mitotic checkpoint complex assembly AuthorsFischer ES, Yu CWH, Hevler JF, McLaughlin SH, Maslen SL, Heck AJR, Freund SMV, Barford D. Journal Nature Communications Oct 26;13(1):6381 (2022)
- Molecular architecture of the augmin complex AuthorsGabel C.A., Li Z., DeMarco A.G., Zhang Z., Yang J., Hall M. C., Barford D., Chang L. Journal Nature Communications 13(1):5449 (2022)
- Structure of the human inner kinetochore bound to a centromeric CENP-A nucleosome AuthorsYatskevich S., Muir K. W., Bellini D., Zhang Z., Yang J., Tischer T., Predin M., Dendooven T., McLaughlin S. H., Barford D. Journal Science 376(6595):844-852 (2022)
- The APC/C targets the Cep152-Cep63 complex at the centrosome to regulate mitotic spindle assembly AuthorsTischer T., Yang J., Barford D. Journal Journal of Cell Science 135(2) (2022)
- Structural basis of human separase regulation by securin and CDK1-cyclin B1 AuthorsJun Yu, Pierre Raia, Chloe M. Ghent, Tobias Raisch, Yashar Sadian, Simone Cavadini, Pramod M. Sabale, David Barford, Stefan Raunser, David O. Morgan, Andreas Boland Journal Nature 596(7870):138-142 (2021)
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Molecular mechanism of Mad1 kinetochore targeting by phosphorylated Bub1
AuthorsFischer E. S., Yu C. W. H., Bellini D., McLaughlin S. H., Orr C. M., Wagner A., Freund S. M. V., Barford D.
Journal
EMBO Reports
22(7):e52242. 5 July 2021 (2021)
Details
DOI10.15252/embr.202052242 PMID34013668 PMCIDPMC8391104 DivisionStructural Studies, Protein & Nucleic Acid Chemistry - Molecular mechanisms of APC/C release from spindle assembly checkpoint inhibition by APC/C SUMOylation AuthorsYatskevich S., Kroonen J. S., Alfieri C., Tischer T., Howes A. C., Clijsters L., Yang J., Zhang Z., Yan K., Vertegaal A. C. O., Barford D. Journal Cell Reports 34(13):108929. 30 March 2021 (2021)
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Structure of the SARS-CoV-2 RNA-dependent RNA polymerase in the presence of favipiravir‑RTP
AuthorsNaydenova K., Muir K. W., Wu L. F., Zhang Z., Coscia F., Peet M. J., Castro-Hartmann P., Qian P., Sader K., Dent K., Kimanius D., Sutherland J. D., Löwe J., Barford D., Russo C. J.
Journal
Proceedings of the National Academy of Sciences of the United States of America
118(7):e2021946118. 16 February 2021 (2021)
Details
DOI10.1073/pnas.2021946118 PMID33526596 PMCIDPMC7896311 DivisionProtein & Nucleic Acid Chemistry, Structural Studies - Crystal structure of the Cenp-HIKHead-TW sub-module of the inner kinetochore CCAN complex AuthorsZhang Z., Bellini D., Barford D. Journal Nucleic Acids Research 48(19):11172-11184. 4 November 2020 (2020)
- Structure of the DOCK2-ELMO1 complex provides insights into regulation of the auto-inhibited state AuthorsChang L., Yang J., Jo C. H., Boland A., Zhang Z., McLaughlin S. H., Abu-Thuraia A., Killoran R. C., Smith M. J., Côté J. F., Barford D. Journal Nature Communications 11(1):3464. 10 July 2020 (2020)
- A unique binding mode of Nek2A to the APC/C allows its ubiquitination during prometaphase AuthorsAlfieri C., Tischer T., Barford D. Journal EMBO Reports 21(6):e49831. 4 June 2020 (2020)
- Structural interconversions of the anaphase-promoting complex/cyclosome (APC/C) regulate cell cycle transitions AuthorsBarford D. Journal Current Opinion in Structural Biology 61:86-97. April 2020 (2020)
- Structure of the inner kinetochore CCAN complex assembled onto a centromeric nucleosome AuthorsYan K., Yang J., Zhang Z., McLaughlin S. H., Chang L., Fasci D., Ehrenhofer-Murray A. E., Heck A. J. R., Barford D. Journal Nature 574(7777):278-282. 10 October 2019 (2019)
- Cyclin A2 degradation during the spindle assembly checkpoint requires multiple binding modes to the APC/C AuthorsZhang S., Tischer T., Barford D. Journal Nature Communications 10(1):3863. 27 August 2019 (2019)
- A MAD way to regulate mitosis AuthorsBarford D. Journal Nature Reviews. Molecular Cell Biology 20(3):135. March 2019 (2019)
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Architecture of the CBF3-centromere complex of the budding yeast kinetochore
AuthorsYan K., Zhang Z., Yang J., McLaughlin S. H., Barford D.
Journal
Nature Structural & Molecular Biology
25(12):1103-1110. December 2018 (2018)
Details
DOI10.1038/s41594-018-0154‑1 PMID30478265 PMCIDPMC6292502 DivisionStructural Studies, Protein & Nucleic Acid Chemistry - Methods for Preparing Cryo-EM Grids of Large Macromolecular Complexes AuthorsChang L., Barford D. Journal Methods in Molecular Biology 1844:209-215. 22 September 2018 (2018)
- Mechanism for remodelling of the cell cycle checkpoint protein MAD2 by the ATPase TRIP13 AuthorsAlfieri C., Chang L., Barford D. Journal Nature 559(7713):274-278. 12 July 2018 (2018)
- The potential of cryo-electron microscopy for structure-based drug design AuthorsBoland A., Chang L., Barford D. Journal Essays in Biochemistry 61(5):543-560. November 2017 (2017)
- Visualizing the complex functions and mechanisms of the anaphase promoting complex/cyclosome (APC/C) AuthorsAlfieri C., Zhang S., Barford D. Journal Open Biology 7(11)170204. November 2017 (2017)
- Cryo-EM structure of a metazoan separase-securin complex at near-atomic resolution AuthorsBoland A., Martin T. G., Zhang Z., Yang J., Bai X. C., Chang L., Scheres S. H., Barford D. Journal Nature Structural & Molecular Biology 24(4):414-418. April 2017 (2017)
- WD40 domain of Apc1 is critical for the coactivator-induced allosteric transition that stimulates APC/C catalytic activity AuthorsLi Q., Chang L., Aibara S., Yang J., Zhang Z., Barford D. Journal Proceedings of the National Academy of Sciences of the United States of America 113(38):10547-10552. 20 September 2016 (2016)
- Molecular basis of APC/C regulation by the spindle assembly checkpoint AuthorsAlfieri C., Chang L., Zhang Z., Yang J., Maslen S., Skehel M., Barford D. Journal Nature 536(7617):431-436. 25 August 2016 (2016)
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Molecular mechanism of APC/C activation by mitotic phosphorylation
AuthorsZhang S., Chang L., Alfieri C., Zhang Z., Yang J., Maslen S., Skehel M., Barford D.
Journal
Nature
533:260-264. 12 May 2016 (2016)
Details
DOI10.1038/nature17973 PMID27120157 PMCIDPMC4878669 DivisionStructural Studies, Cell Biology - Editorial overview: Macromolecular machines and assemblies AuthorsBarford D., Hopfner K. P. Journal Current Opinion in Structural Biology 37:vi-viii. April 2016 (2016)
- Recombinant expression and reconstitution of multiprotein complexes by the USER cloning method in the insect cell-baculovirus expression system AuthorsZhang Z., Yang J., Barford D. Journal Methods 95:13-25. 15 February 2016 (2016)
- Atomic-Resolution Structures of the APC/C Subunits Apc4 and the Apc5 N-Terminal Domain AuthorsCronin N. B., Yang J., Zhang Z., Kulkarni K., Chang L., Yamano H., Barford D. Journal Journal of Molecular Biology 427(20):3300-3315. 9 October 2015 (2015)
- Atomic structure of the APC/C and its mechanism of protein ubiquitination AuthorsChang L., Zhang Z., Yang J., McLaughlin S. H., Barford D. Journal Nature 522(7557):450-454. 25 June 2015 (2015)
- Insights into the anaphase-promoting complex: a molecular machine that regulates mitosis AuthorsChang L., Barford D. Journal Current Opinion in Structural Biology 29:1-9. December 2014 (2014)
- Molecular architecture and mechanism of the anaphase-promoting complex AuthorsChang L., Zhang Z., Yang J., McLaughlin S. H., Barford D. Journal Nature 513(7518):388-393. 18 September 2014 (2014)
- Regulation of A20 and other OTU deubiquitinases by reversible oxidation AuthorsKulathu Y., Garcia F. J., Mevissen T. E., Busch M., Arnaudo N., Carroll K. S., Barford D., Komander D. Journal Nature Communications 4:1569. 5 March 2013 (2013)
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Molecular discrimination of structurally equivalent Lys 63-linked and linear polyubiquitin chains
AuthorsDavid Komander, Francisca Reyes-Turcu, Julien D F Licchesi, Peter Odenwaelder, Keith D Wilkinson, David Barford
Journal
EMBO Reports
10(5):466-473. May 2009 (2009)
Details
DOI10.1038/embor.2009.55 PMID19373254 PMCIDPMC2680876 DivisionProtein & Nucleic Acid Chemistry - Mechanism of multi-site phosphorylation from a ROCK-I:RhoE complex structure AuthorsDavid Komander, Ritu Garg, Paul T. C. Wan, Anne J. Ridley, David Barford Journal EMBO Journal 27(23): 3175-3185 (2008) (2008)